Per-residue counts and mol%, group tallies, Kyte–Doolittle GRAVY score, and ProtParam MW from a sequence. Educational use only.
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Tool usage guide
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Key facts
Category
Education
Input types
textarea, number
Output type
json
Sample coverage
4
API ready
Yes
Overview
Amino Acid Composition Analyzer calculates residue counts and molar percentages from a protein sequence, along with group tallies, Kyte–Doolittle GRAVY hydropathy, and ProtParam molecular weight. Enter a one-letter amino-acid sequence and choose the number of decimal places for the JSON result. Educational use only.
When to use
Check the amino-acid composition and percentage of residues in a protein sequence.
Estimate overall hydrophobicity with the Kyte–Doolittle GRAVY score.
Review residue groups and molecular weight during protein sequence analysis or experiment planning.
How it works
1Enter a one-letter amino-acid sequence; input is case-insensitive.
2Spaces and separators such as *, - and . are ignored.
3The analyzer counts residues, calculates molar percentages and group totals, and computes GRAVY and molecular weight.
4Set decimal places from 0 to 8, then review the structured JSON output.
Use cases
Compare composition and hydropathy across protein constructs or sequence regions.
Check affinity-tag sequences for residue enrichment and overall hydrophilicity.
Perform quick educational calculations for protein biochemistry and laboratory planning.
Examples
1. Inspecting a 6×His affinity tag
Protein laboratory researcher
Background
A researcher wants a quick composition check for the sequence MGSSHHHHHHSSGLVPRGSH before using it as an affinity-tag sequence.
Problem
The sequence contains several histidine and serine residues, so its composition and overall hydropathy need to be quantified.
How to use
Enter `MGSSHHHHHHSSGLVPRGSH` and set Decimal Places to 2.
The 20-residue sequence contains H at 35.00 mol% and S at 25.00 mol%; its GRAVY score is -1.19, indicating a hydrophilic tendency.
2. Checking a poly-alanine sequence
Biochemistry student
Background
A student uses a simple deca-alanine sequence to verify how a uniform residue composition affects hydropathy.
Problem
The student wants to confirm that a sequence made entirely of alanine produces 100% alanine composition and the alanine Kyte–Doolittle value.
How to use
Enter `AAAAAAAAAA` and set Decimal Places to 2.
FAQ
What sequence format does the analyzer accept?
Enter a one-letter amino-acid sequence. Uppercase and lowercase letters are accepted.
Are spaces and separators allowed?
Yes. Spaces and the separators *, - and . are ignored.
What does the GRAVY score show?
GRAVY is the average Kyte–Doolittle hydropathy score. Positive values indicate a hydrophobic tendency, while negative values indicate a hydrophilic tendency.
Can I control the number of decimal places?
Yes. Choose from 0 to 8 decimal places; the default is 2.
How are ambiguous amino-acid codes treated?
B, J, O, U, X and Z are counted separately and excluded from molar percentage calculations.