蛋白-DNA 结合亲和力计算器（Kd 估算）

POST http://127.0.0.1:3003/zh/api/tools/protein-dna-binding-affinity

{
  "mcpServers": {
    "elysiatools-protein-dna-binding-affinity": {
      "name": "protein-dna-binding-affinity",
      "description": "按 1:1 结合平衡从单点数据估算 Kd：当蛋白过量时结合分数 f = [P]÷([P]+Kd)，故 Kd = [P]×(1−f)÷f；f = 0.5 时 Kd 恰等于蛋白浓度。两种输入：直接输入结合分数（0–1），或由荧光各向异性 f = (r−r游离)÷(r结合−r游离) 换算。附带 ΔG° = RT·ln(Kd)（默认 25°C）。判读：<1 nM 极强、1–100 nM 强（典型转录因子/核酸酶靶点）、100 nM–1 µM 中等、>1 µM 弱（查盐浓度、poly(dI-dC) 竞争）。假设：平衡、1:1、蛋白远过量；完整滴定曲线应整体拟合。来源：Cantor & Schimmel、Heyduk & Lee 1990、Lakowicz。仅供教学与实验规划。",
      "baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=protein-dna-binding-affinity",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "protein-dna-binding-affinity",
    "arguments": {
  "mode": "fraction",
  "proteinConcNm": 50,
  "fractionBound": 0.5,
  "anisotropy": 0.18,
  "anisotropyFree": 0.12,
  "anisotropyBound": 0.25,
  "temperatureC": 25,
  "decimalPlaces": 2
}
  }
}