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两种模式配制 DNA/RNA 工作液:目标浓度法按 C1V1 = C2V2 求取样体积 V1 = C2×V2÷C1,并给出补加缓冲液体积;稀释倍数法按 1:X 直接得终浓度 C1÷X 与取样体积 V2÷X。浓度单位只需原液与目标一致(ng/µL、µg/mL、nM 均可),代数与单位无关。内置移液建议:V1 < 0.5 µL 提示改用两步梯度稀释,V1 < 2 µL 提示低吸附枪头;目标高于原液会被拒绝(稀释不能增浓)。来源:Sambrook & Russell 第 3 版、Thermo Fisher 移液技术指南。仅供教学与实验规划。
用三种语言从你的代码中调用此工具。
curl -X POST 'https://api.elysiatools.com/zh/api/tools/nucleic-acid-dilution' \
-H 'Content-Type: application/json' \
-d '{"mode":"c1v1","stockConc":500,"targetConc":100,"dilutionFactor":10,"finalVolumeUl":50,"decimalPlaces":2}'以 JSON 形式 POST 提交输入参数。文件类型参数需先单独上传。
POST https://api.elysiatools.com/zh/api/tools/nucleic-acid-dilution| 参数名 | 类型 | 必填 | 说明 |
|---|---|---|---|
| mode | select | 是 | Solve for the stock volume from a target concentration, or dilute by a fixed 1:X factor. |
| stockConc | number | 是 | Quantified concentration of the stock (any unit — keep it identical to the target unit). |
| targetConc | number | 是 | Desired working concentration (C1V1 = C2V2 mode; must not exceed the stock). |
| dilutionFactor | number | 是 | Total-to-stock ratio of a 1:X dilution (dilution-factor mode; must be greater than 1). |
| finalVolumeUl | number |
将此工具加入你的 Model Context Protocol 服务,让 AI 智能体可以列出并调用它。
将以下内容加入你的 MCP 客户端配置:
{
"mcpServers": {
"elysiatools-nucleic-acid-dilution": {
"name": "nucleic-acid-dilution",
"description": "两种模式配制 DNA/RNA 工作液:目标浓度法按 C1V1 = C2V2 求取样体积 V1 = C2×V2÷C1,并给出补加缓冲液体积;稀释倍数法按 1:X 直接得终浓度 C1÷X 与取样体积 V2÷X。浓度单位只需原液与目标一致(ng/µL、µg/mL、nM 均可),代数与单位无关。内置移液建议:V1 < 0.5 µL 提示改用两步梯度稀释,V1 < 2 µL 提示低吸附枪头;目标高于原液会被拒绝(稀释不能增浓)。来源:Sambrook & Russell 第 3 版、Thermo Fisher 移液技术指南。仅供教学与实验规划。",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=nucleic-acid-dilution",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}连接到 SSE 端点后,列出已开放的工具:
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}通过工具 id 调用,参数由其参数表构建:
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "nucleic-acid-dilution",
"arguments": {
"mode": "c1v1",
"stockConc": 500,
"targetConc": 100,
"dilutionFactor": 10,
"finalVolumeUl": 50,
"decimalPlaces": 2
}
}
}有问题或反馈?请联系 [email protected]
| Total volume of the finished working solution. |
| decimalPlaces | number | 否 | — |
JSON 结果
{
"key": {...},
"metadata": {
"key": "value"
},
"error": "Error message (optional)",
"message": "Notification message (optional)"
}