DNA 摩尔浓度计算器（µg/mL → nM）

POST https://api.elysiatools.com/zh/api/tools/molar-dna-concentration

{
  "mcpServers": {
    "elysiatools-molar-dna-concentration": {
      "name": "molar-dna-concentration",
      "description": "将 DNA 质量浓度换算为摩尔浓度：nM = µg/mL × 10⁶ ÷ (每碱基对质量 × bp)。平均质量按 NEB 惯例：双链 DNA 650 Da/bp、单链 DNA 330 Da/nt（Promega 用 660，同理）。注意常见陷阱：分母若用 10⁻³ 得到的是 µM 而非 nM（差 1000 倍）。1 µg/mL = 1 ng/µL 两种单位通用；输入体积即可得总 pmol 与每 µL 分子数（衔接 dna-copy-number 工具）。来源：NEB NEBioCalculator、Promega BioMath、Avogadro 常数 6.022×10²³。仅供教学与实验规划。",
      "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=molar-dna-concentration",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "molar-dna-concentration",
    "arguments": {
  "concentration": 50,
  "dnaType": "dsDNA",
  "lengthBp": 1000,
  "sampleVolumeUl": 100,
  "decimalPlaces": 3
}
  }
}