DNA Molar Concentration Calculator (µg/mL → nM)

DNA Molar Concentration Calculator (µg/mL → nM) | Online Free Tool | Elysia Tools

Tool usage guide

Learn when to use this tool, what it supports, and how real users apply it.

Key facts

Category: Education
Input types: number, select
Output type: json
Sample coverage: 4
API ready: Yes

Overview

Convert DNA mass concentration in µg/mL (numerically equal to ng/µL) into nM, µM, and pmol/µL using average molecular masses for dsDNA or ssDNA. Enter the DNA length and optionally the sample volume to calculate total pmol and molecule counts. This calculator is intended for education and experimental planning.

When to use

Convert a measured DNA concentration from µg/mL or ng/µL to nM before setting up a molecular biology reaction.
Calculate molar amounts for PCR fragments, inserts, plasmids, or ssDNA based on DNA length and type.
Estimate total pmol and DNA molecule counts when the sample volume is known.

How it works

1Enter the DNA concentration in µg/mL; this value is numerically identical to ng/µL.
2Select dsDNA, using 650 Da/bp, or ssDNA, using 330 Da/nt.
3Enter the full DNA length in bp or nt.
4Optionally enter the sample volume to calculate total pmol; the results also report µM, pmol/µL, and molecule counts.

Use cases

Convert purified PCR fragment concentrations into molar concentrations for reaction planning.
Compare molar amounts of dsDNA and ssDNA molecules with different lengths.
Estimate total DNA quantity and molecule counts in a measured sample volume.

Examples

1. 1000 bp dsDNA fragment at 50 µg/mL

Molecular biology researcher

Background

A 1000 bp double-stranded PCR fragment is measured at 50 µg/mL, which is also 50 ng/µL.

Problem

The researcher needs the molar concentration and total amount available in a 100 µL sample.

How to use

Enter concentration 50, select dsDNA, enter length 1000, set sample volume to 100 µL, and choose 3 decimal places.

{"concentration":50,"dnaType":"dsDNA","lengthBp":1000,"sampleVolumeUl":100,"decimalPlaces":3}

Outcome

The result is 76.923 nM, 0.077 µM, and 0.077 pmol/µL. The 100 µL sample contains 7.692 pmol, approximately 4.63 × 10¹² molecules.

2. 76-nt ssDNA preparation at 20 µg/mL

Research scientist

Background

A 76-nucleotide single-stranded DNA preparation has a concentration of 20 µg/mL.

Problem

The scientist needs to use the ssDNA molecular-mass convention rather than the dsDNA value.

How to use

Enter concentration 20, select ssDNA, enter length 76, leave sample volume at 0, and choose 2 decimal places.

FAQ

What formula does the calculator use?

It uses nM = (µg/mL × 10⁶) ÷ (Da per bp or nt × DNA length).

What mass value is used for dsDNA?

The calculator uses an average molecular mass of 650 Da per base pair.

What mass value is used for ssDNA?

The calculator uses an average molecular mass of 330 Da per nucleotide.

Is 1 µg/mL equal to 1 ng/µL?

Yes. These concentration units are numerically identical.

What happens if I leave sample volume at zero?

The calculator skips the total-pmol calculation while still reporting concentration-based results.

{"concentration":20,"dnaType":"ssDNA","lengthBp":76,"sampleVolumeUl":0,"decimalPlaces":2}

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Examples that match this tool

Continue with connected tools and hubs

Learn when to use this tool, what it supports, and how real users apply it.

Key facts

Overview

When to use

How it works

Use cases

Examples

1. 1000 bp dsDNA fragment at 50 µg/mL

2. 76-nt ssDNA preparation at 20 µg/mL

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