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将 OD600 浊度读数换算为细胞浓度与细胞干重（CDW）：归一化 OD = 读数 × 稀释倍数 ÷ 光程；cells/mL = OD × 每 OD 细胞数（默认 1×10⁹，E. coli 常用值）；CDW g/L = OD × 每 OD 干重（默认 0.36，BioNumbers 109837，文献范围 0.3–0.5）。两个系数均可编辑——菌株、培养基与仪器都会影响换算；默认组合自洽：0.36 g/L ÷ 10⁹ cells/mL = 0.36 pg/细胞。OD > 1 线性区外会标记提示稀释复测（Sigma-Aldrich）。来源：BioNumbers 109835/109837、Agilent/VectorBuilder、Sigma-Aldrich。建议用自测 OD-CFU 或干重曲线校准。仅供教学与实验规划。
用三种语言从你的代码中调用此工具。
curl -X POST 'https://api.elysiatools.com/zh/api/tools/od600-cell-density' \
-H 'Content-Type: application/json' \
-d '{"od600":0.5,"dilutionFactor":1,"pathLengthCm":1,"cellsPerOd":1000000000,"cdwPerOd":0.36,"cultureVolumeMl":25,"decimalPlaces":4}'以 JSON 形式 POST 提交输入参数。文件类型参数需先单独上传。
POST https://api.elysiatools.com/zh/api/tools/od600-cell-density| 参数名 | 类型 | 必填 | 说明 |
|---|---|---|---|
| od600 | number | 是 | Raw absorbance of the (diluted) sample at 600 nm, blanked against medium. |
| dilutionFactor | number | 是 | Sample-to-total dilution before measurement (1 = measured undiluted). |
| pathLengthCm | number | 是 | Cuvette path length (1 cm standard; readings are normalized to 1 cm). |
| cellsPerOd | number | 是 | Viable+total cells per mL at OD600 = 1 (E. coli default 1×10⁹; range 5×10⁸–1.2×10⁹). |
| cdwPerOd | number | 是 |
将此工具加入你的 Model Context Protocol 服务，让 AI 智能体可以列出并调用它。
将以下内容加入你的 MCP 客户端配置：
{
"mcpServers": {
"elysiatools-od600-cell-density": {
"name": "od600-cell-density",
"description": "将 OD600 浊度读数换算为细胞浓度与细胞干重（CDW）：归一化 OD = 读数 × 稀释倍数 ÷ 光程；cells/mL = OD × 每 OD 细胞数（默认 1×10⁹，E. coli 常用值）；CDW g/L = OD × 每 OD 干重（默认 0.36，BioNumbers 109837，文献范围 0.3–0.5）。两个系数均可编辑——菌株、培养基与仪器都会影响换算；默认组合自洽：0.36 g/L ÷ 10⁹ cells/mL = 0.36 pg/细胞。OD > 1 线性区外会标记提示稀释复测（Sigma-Aldrich）。来源：BioNumbers 109835/109837、Agilent/VectorBuilder、Sigma-Aldrich。建议用自测 OD-CFU 或干重曲线校准。仅供教学与实验规划。",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=od600-cell-density",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}连接到 SSE 端点后，列出已开放的工具：
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}通过工具 id 调用，参数由其参数表构建：
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "od600-cell-density",
"arguments": {
"od600": 0.5,
"dilutionFactor": 1,
"pathLengthCm": 1,
"cellsPerOd": 1000000000,
"cdwPerOd": 0.36,
"cultureVolumeMl": 25,
"decimalPlaces": 4
}
}
}有问题或反馈？请联系 [email protected]
| Grams of cell dry weight per liter at OD600 = 1 (default 0.36; literature 0.3–0.5). |
| cultureVolumeMl | number | 否 | Total culture volume in mL; enter 0 to skip total cells and total CDW. |
| decimalPlaces | number | 否 | — |
JSON 结果
{
"key": {...},
"metadata": {
"key": "value"
},
"error": "Error message (optional)",
"message": "Notification message (optional)"
}