1. Calculating pH of a Phosphate Buffer
Biochemistry ResearcherBackground
A researcher needs to verify the pH of a phosphate buffer prepared with equal concentrations of dihydrogen phosphate and hydrogen phosphate.
Problem
Determine the exact pH of a solution containing 0.1 M acid and 0.1 M conjugate base.
How to use
Set the mode to 'Find pH from acid/base ratio', select the 'Phosphate' buffer system, enter 0.1 for both acid and base concentrations, and set the temperature to 25 °C.
Mode: Find pH, Buffer System: Phosphate, Acid Concentration: 0.1 mol/L, Base Concentration: 0.1 mol/LOutcome
The calculator outputs a pH of 7.210, confirming that an equimolar ratio matches the pKa of the phosphate system.