1. Preparing a 1:5 Total-Volume Solution
Lab TechnicianBackground
A biological assay protocol calls for a 1:5 sample dilution using phosphate-buffered saline to make 100 mL of working solution.
Problem
Determine exact solute and buffer volumes under standard total-volume convention.
How to use
Set Input Notation to 'Ratio 1:X', enter 5 as Value, select 'total' convention, and enter 100 with unit 'mL'.
mode: ratio, value: 5, convention: total, finalVolume: 100, volumeUnit: mL, decimalPlaces: 4Outcome
Converts to fraction 1/5 (20%, 5-fold dilution) and specifies mixing 20 mL solute with 80 mL diluent.