Education
Nucleic Acid Dilution Calculator (C1V1 = C2V2)
DNA/RNA working-solution prep: C1V1 = C2V2 mode solves the stock volume V1 = C2V2/C1 plus diluent; 1:X mode gives final concentration C1/X and volumes. Unit-free algebra, pipetting safety advice (<0.5 µL suggests serial dilution), and impossible targets rejected. Sambrook & Russell / Thermo Fisher derived. Educational use only.
json· JSON resultOpen tool
Quick start
Call this tool from your code in three languages.
cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/nucleic-acid-dilution' \
-H 'Content-Type: application/json' \
-d '{"mode":"c1v1","stockConc":500,"targetConc":100,"dilutionFactor":10,"finalVolumeUl":50,"decimalPlaces":2}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
HTTP
POST https://api.elysiatools.com/en/api/tools/nucleic-acid-dilutionParameters
| Name | Type | Required | Description |
|---|---|---|---|
| mode | select | Yes | Solve for the stock volume from a target concentration, or dilute by a fixed 1:X factor. |
| stockConc | number | Yes | Quantified concentration of the stock (any unit — keep it identical to the target unit). |
| targetConc | number | Yes | Desired working concentration (C1V1 = C2V2 mode; must not exceed the stock). |
| dilutionFactor | number | Yes | Total-to-stock ratio of a 1:X dilution (dilution-factor mode; must be greater than 1). |
| finalVolumeUl |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
mcp.json
{
"mcpServers": {
"elysiatools-nucleic-acid-dilution": {
"name": "nucleic-acid-dilution",
"description": "DNA/RNA working-solution prep: C1V1 = C2V2 mode solves the stock volume V1 = C2V2/C1 plus diluent; 1:X mode gives final concentration C1/X and volumes. Unit-free algebra, pipetting safety advice (<0.5 µL suggests serial dilution), and impossible targets rejected. Sambrook & Russell / Thermo Fisher derived. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=nucleic-acid-dilution",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
tools/list
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
tools/call
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "nucleic-acid-dilution",
"arguments": {
"mode": "c1v1",
"stockConc": 500,
"targetConc": 100,
"dilutionFactor": 10,
"finalVolumeUl": 50,
"decimalPlaces": 2
}
}
}Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).