Education

DNA Molar Concentration Calculator (µg/mL → nM)

Mass-to-molar DNA conversion: nM = (µg/mL × 10⁶)/(Da-per-bp × bp) with 650 Da/bp dsDNA and 330 Da/nt ssDNA, plus µM, pmol/µL, total pmol, and molecules/µL via Avogadro. Warns about the µM-vs-nM 1000× slip. NEB NEBioCalculator / Promega BioMath derived. Educational use only.

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Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'https://api.elysiatools.com/en/api/tools/molar-dna-concentration' \
  -H 'Content-Type: application/json' \
  -d '{"concentration":50,"dnaType":"dsDNA","lengthBp":1000,"sampleVolumeUl":100,"decimalPlaces":3}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST https://api.elysiatools.com/en/api/tools/molar-dna-concentration

Parameters

Name	Type	Required	Description
concentration	number	Yes	Mass concentration of the DNA sample. 1 µg/mL is numerically identical to 1 ng/µL.
dnaType	select	Yes	dsDNA averages 650 Da/bp; ssDNA averages 330 Da/nt (NEB convention).
lengthBp	number	Yes	Full length of the DNA molecule in base pairs (nt for ssDNA).
sampleVolumeUl	number	No	Volume of the sample in µL; enter 0 to skip the total-pmol calculation.
decimalPlaces	number

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-molar-dna-concentration": {
      "name": "molar-dna-concentration",
      "description": "Mass-to-molar DNA conversion: nM = (µg/mL × 10⁶)/(Da-per-bp × bp) with 650 Da/bp dsDNA and 330 Da/nt ssDNA, plus µM, pmol/µL, total pmol, and molecules/µL via Avogadro. Warns about the µM-vs-nM 1000× slip. NEB NEBioCalculator / Promega BioMath derived. Educational use only.",
      "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=molar-dna-concentration",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "molar-dna-concentration",
    "arguments": {
  "concentration": 50,
  "dnaType": "dsDNA",
  "lengthBp": 1000,
  "sampleVolumeUl": 100,
  "decimalPlaces": 3
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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