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Enzyme Activity Unit Calculator (U, µmol/min)

Converts product-amount or ΔA/min assay data into enzyme units: 1 U = 1 µmol/min = 16.67 nkat, with Beer–Lambert absorbance conversion and linear-window checks. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/enzyme-activity-unit' \
  -H 'Content-Type: application/json' \
  -d '{"mode":"amount","productUmol":6,"timeMin":3,"deltaAPerMin":0.622,"extinctionCoeff":6.22,"pathLength":1,"volumeMl":1,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP
POST https://api.elysiatools.com/en/api/tools/enzyme-activity-unit

Parameters

NameTypeRequiredDescription
modeselectYesEnter product amount and time, or convert an absorbance slope.
productUmolnumberYesProduct formed during the assay (amount mode).
timeMinnumberYesTime over which the product accumulated (amount mode).
deltaAPerMinnumberYesInitial linear slope of the absorbance trace (absorbance mode).
extinctionCoeffnumberYes

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json
{
  "mcpServers": {
    "elysiatools-enzyme-activity-unit": {
      "name": "enzyme-activity-unit",
      "description": "Converts product-amount or ΔA/min assay data into enzyme units: 1 U = 1 µmol/min = 16.67 nkat, with Beer–Lambert absorbance conversion and linear-window checks. Educational use only.",
      "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=enzyme-activity-unit",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list
{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call
{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "enzyme-activity-unit",
    "arguments": {
  "mode": "amount",
  "productUmol": 6,
  "timeMin": 3,
  "deltaAPerMin": 0.622,
  "extinctionCoeff": 6.22,
  "pathLength": 1,
  "volumeMl": 1,
  "decimalPlaces": 2
}
  }
}
Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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Millimolar extinction coefficient of the monitored species (NADH: 6.22 at 340 nm).
pathLengthnumberYesOptical path length of the cuvette or microplate well (absorbance mode).
volumeMlnumberYesTotal volume in the light path (absorbance mode).
decimalPlacesnumberNo—

Response format

JSON result

JSON
{
  "key": {...},
  "metadata": {
    "key": "value"
  },
  "error": "Error message (optional)",
  "message": "Notification message (optional)"
}