Education

Bradford Assay Calculator (BSA Standard Curve)

Least-squares BSA curve, back-calculated unknowns with dilution factor, extrapolation flags, and protein-specific dye-response caveats. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'http://127.0.0.1:3003/en/api/tools/bradford-assay-calculator' \
  -H 'Content-Type: application/json' \
  -d '{"standards":"0, 0.000\n10, 0.200\n20, 0.400\n40, 0.800","unknowns":"0.50\n0.25","dilutionFactor":1,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST http://127.0.0.1:3003/en/api/tools/bradford-assay-calculator

Parameters

Name	Type	Required	Description
standards	textarea	Yes	One standard per line: concentration in µg/mL, then A595 (comma, semicolon, tab, or space separated).
unknowns	textarea	Yes	One sample A595 reading per line (blank-subtracted readings are fine as long as the curve includes the blank).
dilutionFactor	number	Yes	Dilution applied to the sample before the assay (1 = undiluted).
decimalPlaces	number	No	—

Response format

JSON result

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-bradford-assay-calculator": {
      "name": "bradford-assay-calculator",
      "description": "Least-squares BSA curve, back-calculated unknowns with dilution factor, extrapolation flags, and protein-specific dye-response caveats. Educational use only.",
      "baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=bradford-assay-calculator",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "bradford-assay-calculator",
    "arguments": {
  "standards": "0, 0.000\n10, 0.200\n20, 0.400\n40, 0.800",
  "unknowns": "0.50\n0.25",
  "dilutionFactor": 1,
  "decimalPlaces": 2
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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