1. Textbook BSA curve with two unknowns
Biochemistry studentBackground
A student has four BSA standards with concentrations of 0, 10, 20, and 40 µg/mL and proportional A595 readings.
Problem
They need to convert unknown absorbances of 0.50 and 0.25 into protein concentrations.
How to use
Enter the standards, add the two unknown A595 readings, set the dilution factor to 1, and choose two decimal places.
Standards: 0, 0.000; 10, 0.200; 20, 0.400; 40, 0.800. Unknowns: 0.50 and 0.25. Dilution factor: 1.Outcome
The fitted line has a slope of 0.02, an intercept of 0, and R² = 1.00. The unknowns are reported as 25.00 and 12.50 µg/mL, both within the standard range.