GFP reporter transfection scoring: efficiency = GFP+ / total × 100 with a Wilson 95% CI in single mode and per-replicate mean ± SD in replicate mode. Interpretation bands from excellent (≥70%) to low (<10%) with optimization hints. Wilson 1927 / Newcombe 1998 / Kim & Eberwine 2010 derived. Educational use only.
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Tool usage guide
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Key facts
Category
Education
Input types
select, number, text
Output type
json
Sample coverage
4
API ready
Yes
Overview
The Transfection Efficiency Calculator estimates GFP-based transfection efficiency from GFP-positive and total cell counts. Use single mode for one pooled measurement with a Wilson 95% confidence interval, or replicate mode for per-replicate percentages, mean, and sample standard deviation. Educational use only.
When to use
Calculate the GFP-positive percentage from a single flow cytometry or microscopy count.
Summarize transfection efficiency across replicate wells or fluorescence microscopy fields.
Review efficiency results with a confidence interval or replicate variability before comparing experiments.
How it works
1Select Single measurement or Replicate wells or fields.
2In single mode, enter total cells counted and GFP-positive cells.
3In replicate mode, enter comma-separated total and GFP-positive counts in matching order.
4Choose the decimal places and review the calculated efficiency and statistics.
Use cases
Evaluate GFP reporter expression after a transfection experiment.
Summarize cell counts from replicate wells or randomly selected microscopy fields.
Compare observed GFP-positive rates with broad transfection-efficiency interpretation bands.
Examples
1. Flow cytometry: 150 GFP-positive cells out of 500
Cell biology researcher
Background
A researcher counts 500 viable single-cell events after GFP-plasmid transfection and identifies 150 cells above the GFP threshold.
Problem
Calculate the pooled transfection efficiency and quantify uncertainty around the percentage.
How to use
Select Single measurement, enter 500 for total cells and 150 for GFP-positive cells, then set decimal places to 2.