Amino Acid Composition Analyzer

Amino Acid Composition Analyzer | Online Free Tool | Elysia Tools

Tool usage guide

Learn when to use this tool, what it supports, and how real users apply it.

Key facts

Category: Education
Input types: textarea, number
Output type: json
Sample coverage: 4
API ready: Yes

Overview

Amino Acid Composition Analyzer calculates residue counts and molar percentages from a protein sequence, along with group tallies, Kyte–Doolittle GRAVY hydropathy, and ProtParam molecular weight. Enter a one-letter amino-acid sequence and choose the number of decimal places for the JSON result. Educational use only.

When to use

Check the amino-acid composition and percentage of residues in a protein sequence.
Estimate overall hydrophobicity with the Kyte–Doolittle GRAVY score.
Review residue groups and molecular weight during protein sequence analysis or experiment planning.

How it works

1Enter a one-letter amino-acid sequence; input is case-insensitive.
2Spaces and separators such as *, - and . are ignored.
3The analyzer counts residues, calculates molar percentages and group totals, and computes GRAVY and molecular weight.
4Set decimal places from 0 to 8, then review the structured JSON output.

Use cases

Compare composition and hydropathy across protein constructs or sequence regions.
Check affinity-tag sequences for residue enrichment and overall hydrophilicity.
Perform quick educational calculations for protein biochemistry and laboratory planning.

Examples

1. Inspecting a 6×His affinity tag

Protein laboratory researcher

Background

A researcher wants a quick composition check for the sequence MGSSHHHHHHSSGLVPRGSH before using it as an affinity-tag sequence.

Problem

The sequence contains several histidine and serine residues, so its composition and overall hydropathy need to be quantified.

How to use

Enter `MGSSHHHHHHSSGLVPRGSH` and set Decimal Places to 2.

{"sequence":"MGSSHHHHHHSSGLVPRGSH","decimalPlaces":2}

Outcome

The 20-residue sequence contains H at 35.00 mol% and S at 25.00 mol%; its GRAVY score is -1.19, indicating a hydrophilic tendency.

2. Checking a poly-alanine sequence

Biochemistry student

Background

A student uses a simple deca-alanine sequence to verify how a uniform residue composition affects hydropathy.

Problem

The student wants to confirm that a sequence made entirely of alanine produces 100% alanine composition and the alanine Kyte–Doolittle value.

How to use

Enter `AAAAAAAAAA` and set Decimal Places to 2.

FAQ

What sequence format does the analyzer accept?

Enter a one-letter amino-acid sequence. Uppercase and lowercase letters are accepted.

Are spaces and separators allowed?

Yes. Spaces and the separators *, - and . are ignored.

What does the GRAVY score show?

GRAVY is the average Kyte–Doolittle hydropathy score. Positive values indicate a hydrophobic tendency, while negative values indicate a hydrophilic tendency.

Can I control the number of decimal places?

Yes. Choose from 0 to 8 decimal places; the default is 2.

How are ambiguous amino-acid codes treated?

B, J, O, U, X and Z are counted separately and excluded from molar percentage calculations.

{"sequence":"AAAAAAAAAA","decimalPlaces":2}

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Learn when to use this tool, what it supports, and how real users apply it.

Key facts

Overview

When to use

How it works

Use cases

Examples

1. Inspecting a 6×His affinity tag

2. Checking a poly-alanine sequence

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