DNA Copy Number Calculator (Concentration → Copies) | Online Free Tool | Elysia Tools
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DNA Copy Number Calculator (Concentration → Copies)
Convert DNA mass concentration to copies/µL via copies = c(g/µL) × Avogadro ÷ (bp × 660), with ng/µL–pg/µL–µg/µL units, pmol/µL output, total copies per volume, and A260-overestimation caveats. Lab-planning use only.
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Key facts
Category
Education
Input types
number, select
Output type
json
Sample coverage
4
API ready
Yes
Overview
The DNA Copy Number Calculator converts DNA concentration and template length into copies per µL using the average molecular weight of 660 g/mol per base pair and Avogadro’s constant. It also reports pmol/µL and, when a sample volume is entered, the total number of DNA copies.
When to use
Estimate plasmid, genome, or amplicon copies per µL from a measured DNA concentration.
Calculate how many template molecules are present in a selected sample volume.
Prepare DNA standards or plan PCR and qPCR template amounts.
How it works
1Enter the DNA concentration and select ng/µL, pg/µL, µg/mL, or µg/µL.
2Enter the total double-stranded template length in base pairs.
3Optionally enter the template volume in µL to calculate total copies.
4The calculator applies copies = concentration in g/µL × Avogadro’s constant ÷ (base pairs × 660) and returns copies/µL and pmol/µL.
Use cases
Convert plasmid miniprep measurements into template copies for PCR or qPCR planning.
Estimate molecular copies in lambda DNA or other long-template standards.
Compare copy concentrations across DNA samples reported in different mass units.
Examples
1. Plasmid miniprep copy estimate
Molecular biology researcher
Background
A plasmid miniprep has a measured concentration of 50 ng/µL and a total length of 5,000 bp.
Problem
The researcher needs the molecular copy concentration and the total copies in a 2 µL template volume.
How to use
Enter 50 for concentration, select ng/µL, enter 5000 bp, and enter 2 µL as the sample volume.
The result is 9.12×10⁹ copies/µL, 0.0152 pmol/µL, and 1.82×10¹⁰ total copies in 2 µL.
2. Lambda DNA standard calculation
Assay development scientist
Background
A lambda DNA working solution is measured at 10 ng/µL, and lambda DNA has a length of 48,502 bp.
Problem
The scientist wants to determine the copy concentration without calculating total copies for a specific volume.
How to use
Enter 10 for concentration, select ng/µL, enter 48502 bp, and leave the sample volume at 0.
FAQ
What does this DNA calculator calculate?
It converts DNA mass concentration into molecular copies per µL, pmol/µL, and optionally total copies in a specified volume.
Which concentration units are supported?
You can enter ng/µL, pg/µL, µg/mL, or µg/µL.
What template length should I enter?
Enter the total double-stranded length in base pairs, such as a plasmid size, genome size, or amplicon length.
How is total copy number calculated?
The calculator multiplies the copies per µL result by the optional sample volume in µL. Enter 0 to skip this calculation.
Is the result suitable for exact oligonucleotide quantification?
No. The 660 Da per base-pair value is an average intended for plasmids, genomes, and amplicons; exact oligonucleotides require base-specific mass calculations.