1. Converting OD600 Absorbance to Percent Transmittance
MicrobiologistBackground
A lab researcher measures an OD600 reading of 1.0 for a bacterial culture in a cuvette.
Problem
Determine the exact percentage of light passing through the suspension.
How to use
Enter 1 as the Value, select 'Absorbance (A)' as the Input Quantity, and set Decimal Places to 4.
{"value": 1, "inputQuantity": "absorbance", "decimalPlaces": 4}Outcome
Returns T = 0.1 and %T = 10%, confirming 10% light transmission.