Education

PCR Cycle Number / Product Amount Calculator (2^n)

PCR product from ideal exponential amplification copies = template × (1 + e)^n (2^n at 100%), with ng conversion from amplicon length, plateau flag (~10¹²–10¹³ molecules), and >35-cycle warning. Derived from Saiki 1988, Innis & Gelfand 1990, Lorenz 2012. Lab-planning use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'http://127.0.0.1:3003/en/api/tools/pcr-cycle-number' \
  -H 'Content-Type: application/json' \
  -d '{"templateCopies":1000,"cycles":30,"efficiencyPercent":100,"ampliconLengthBp":500,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST http://127.0.0.1:3003/en/api/tools/pcr-cycle-number

Parameters

Name	Type	Required	Description
templateCopies	number	Yes	Starting number of target molecules (see the companion copy-number tool).
cycles	number	Yes	Number of thermal cycles (most protocols: 25–35).
efficiencyPercent	number	Yes	Per-cycle amplification efficiency; 100% = perfect doubling, real PCR typically 70–100%.
ampliconLengthBp	number	No	Amplicon length in base pairs for the ng-yield conversion; enter 0 to skip.
decimalPlaces	number

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-pcr-cycle-number": {
      "name": "pcr-cycle-number",
      "description": "PCR product from ideal exponential amplification copies = template × (1 + e)^n (2^n at 100%), with ng conversion from amplicon length, plateau flag (~10¹²–10¹³ molecules), and >35-cycle warning. Derived from Saiki 1988, Innis & Gelfand 1990, Lorenz 2012. Lab-planning use only.",
      "baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=pcr-cycle-number",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "pcr-cycle-number",
    "arguments": {
  "templateCopies": 1000,
  "cycles": 30,
  "efficiencyPercent": 100,
  "ampliconLengthBp": 500,
  "decimalPlaces": 2
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

Browse tools Open tool