Education

Passage Dilution Ratio Calculator (1:N)

1:N split from current and target densities, with keep/fresh-medium volumes and inter-passage doublings. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'http://127.0.0.1:3003/en/api/tools/passage-dilution-ratio' \
  -H 'Content-Type: application/json' \
  -d '{"mode":"volume","currentDensity":2000000,"targetDensity":200000,"cultureVolumeMl":10,"intervalHours":48,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST http://127.0.0.1:3003/en/api/tools/passage-dilution-ratio

Parameters

Name	Type	Required	Description
mode	select	Yes	Just the 1:N ratio, or the split volumes for a specific culture volume.
currentDensity	number	Yes	Viable-cell density of the culture being passaged (from a hemocytometer count).
targetDensity	number	Yes	Seed density you want in the new flask.
cultureVolumeMl	number	Yes	Total volume of the culture being split (volume mode).
intervalHours	number

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-passage-dilution-ratio": {
      "name": "passage-dilution-ratio",
      "description": "1:N split from current and target densities, with keep/fresh-medium volumes and inter-passage doublings. Educational use only.",
      "baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=passage-dilution-ratio",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "passage-dilution-ratio",
    "arguments": {
  "mode": "volume",
  "currentDensity": 2000000,
  "targetDensity": 200000,
  "cultureVolumeMl": 10,
  "intervalHours": 48,
  "decimalPlaces": 2
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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