Education
OD600 Cell Density Calculator (E. coli)
OD600 turbidity to cells/mL (1×10⁹ per OD default) and cell dry weight g/L (0.36 per OD, BioNumbers 109837), normalized for dilution factor and path length, with total cells/CDW from the culture volume, per-cell pg CDW, and an OD > 1 linearity flag. Editable strain-specific factors. Agilent/VectorBuilder/BioNumbers derived. Educational use only.
Quick start
Call this tool from your code in three languages.
curl -X POST 'https://api.elysiatools.com/en/api/tools/od600-cell-density' \
-H 'Content-Type: application/json' \
-d '{"od600":0.5,"dilutionFactor":1,"pathLengthCm":1,"cellsPerOd":1000000000,"cdwPerOd":0.36,"cultureVolumeMl":25,"decimalPlaces":4}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
POST https://api.elysiatools.com/en/api/tools/od600-cell-densityParameters
| Name | Type | Required | Description |
|---|---|---|---|
| od600 | number | Yes | Raw absorbance of the (diluted) sample at 600 nm, blanked against medium. |
| dilutionFactor | number | Yes | Sample-to-total dilution before measurement (1 = measured undiluted). |
| pathLengthCm | number | Yes | Cuvette path length (1 cm standard; readings are normalized to 1 cm). |
| cellsPerOd | number | Yes | Viable+total cells per mL at OD600 = 1 (E. coli default 1×10⁹; range 5×10⁸–1.2×10⁹). |
| cdwPerOd | number |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
{
"mcpServers": {
"elysiatools-od600-cell-density": {
"name": "od600-cell-density",
"description": "OD600 turbidity to cells/mL (1×10⁹ per OD default) and cell dry weight g/L (0.36 per OD, BioNumbers 109837), normalized for dilution factor and path length, with total cells/CDW from the culture volume, per-cell pg CDW, and an OD > 1 linearity flag. Editable strain-specific factors. Agilent/VectorBuilder/BioNumbers derived. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=od600-cell-density",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "od600-cell-density",
"arguments": {
"od600": 0.5,
"dilutionFactor": 1,
"pathLengthCm": 1,
"cellsPerOd": 1000000000,
"cdwPerOd": 0.36,
"cultureVolumeMl": 25,
"decimalPlaces": 4
}
}
}