Education

OD260/280 Nucleic Acid Purity Ratio Calculator

Judge DNA/RNA purity from A260/A280 (pure DNA ~1.8, pure RNA ~2.0; low = protein/phenol contamination, DNA > 2.0 = RNA contamination) plus the optional A260/A230 secondary check. Derived from Manchester 1995, Sambrook & Russell, Thermo Fisher T042. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'http://127.0.0.1:3003/en/api/tools/od260-280-ratio' \
  -H 'Content-Type: application/json' \
  -d '{"nucleicAcidType":"DNA","a260":0.45,"a280":0.25,"a230":0.2,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST http://127.0.0.1:3003/en/api/tools/od260-280-ratio

Parameters

Name	Type	Required	Description
nucleicAcidType	select	Yes	Expected ratio: DNA ~1.8, RNA ~2.0 — the acceptable window shifts with type.
a260	number	Yes	Absorbance at 260 nm (nucleic acid peak), same diluted sample as the A280 reading.
a280	number	Yes	Absorbance at 280 nm (protein/phenol peak) of the same diluted sample.
a230	number	No	Absorbance at 230 nm; enter 0 to skip the secondary A260/A230 ratio.
decimalPlaces	number

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-od260-280-ratio": {
      "name": "od260-280-ratio",
      "description": "Judge DNA/RNA purity from A260/A280 (pure DNA ~1.8, pure RNA ~2.0; low = protein/phenol contamination, DNA > 2.0 = RNA contamination) plus the optional A260/A230 secondary check. Derived from Manchester 1995, Sambrook & Russell, Thermo Fisher T042. Educational use only.",
      "baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=od260-280-ratio",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "od260-280-ratio",
    "arguments": {
  "nucleicAcidType": "DNA",
  "a260": 0.45,
  "a280": 0.25,
  "a230": 0.2,
  "decimalPlaces": 2
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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