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Catalytic Efficiency Calculator (kcat/Km)

kcat/Km in M⁻¹s⁻¹ from direct kcat or from Vmax/[E], with scientific-notation output and bands up to the diffusion-controlled perfection limit. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/enzyme-catalytic-efficiency' \
  -H 'Content-Type: application/json' \
  -d '{"mode":"direct","kcatValue":1000,"kcatUnit":"per-sec","kmValue":100,"kmUnit":"micro","vmaxUMLMin":50,"enzymeUM":2,"decimalPlaces":4}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP
POST https://api.elysiatools.com/en/api/tools/enzyme-catalytic-efficiency

Parameters

NameTypeRequiredDescription
modeselectYesEnter kcat directly, or derive it from Vmax and enzyme concentration.
kcatValuenumberYesTurnover number in the unit selected below (direct mode).
kcatUnitselectYesWhether kcat is per second or per minute.
kmValuenumberYesMichaelis constant in the unit selected below.
kmUnitselectYes

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json
{
  "mcpServers": {
    "elysiatools-enzyme-catalytic-efficiency": {
      "name": "enzyme-catalytic-efficiency",
      "description": "kcat/Km in M⁻¹s⁻¹ from direct kcat or from Vmax/[E], with scientific-notation output and bands up to the diffusion-controlled perfection limit. Educational use only.",
      "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=enzyme-catalytic-efficiency",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list
{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call
{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "enzyme-catalytic-efficiency",
    "arguments": {
  "mode": "direct",
  "kcatValue": 1000,
  "kcatUnit": "per-sec",
  "kmValue": 100,
  "kmUnit": "micro",
  "vmaxUMLMin": 50,
  "enzymeUM": 2,
  "decimalPlaces": 4
}
  }
}
Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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Concentration unit of Km.
vmaxUMLMinnumberYesMaximal velocity as a concentration rate (from-Vmax mode).
enzymeUMnumberYesActive-site concentration (from-Vmax mode).
decimalPlacesnumberNo—

Response format

JSON result

JSON
{
  "key": {...},
  "metadata": {
    "key": "value"
  },
  "error": "Error message (optional)",
  "message": "Notification message (optional)"
}