Education

BCA Assay Calculator (Protein Standard Curve)

Least-squares A562 curve fit, back-calculated unknowns with dilution factor, extrapolation flags, and detergent/reducing-agent compatibility notes. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'https://api.elysiatools.com/en/api/tools/bca-assay-calculator' \
  -H 'Content-Type: application/json' \
  -d '{"standards":"0, 0.000\n200, 0.200\n500, 0.500\n1000, 1.000\n2000, 2.000","unknowns":"0.735\n1.640","dilutionFactor":1,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST https://api.elysiatools.com/en/api/tools/bca-assay-calculator

Parameters

Name	Type	Required	Description
standards	textarea	Yes	One standard per line: concentration in µg/mL, then A562 (comma, semicolon, tab, or space separated).
unknowns	textarea	Yes	One sample A562 reading per line.
dilutionFactor	number	Yes	Dilution applied to the sample before the assay (1 = undiluted).
decimalPlaces	number	No	—

Response format

JSON result

JSON

{
  "key": {...},
  "metadata": {
    "key": "value"
  },
  "error": "Error message (optional)",
  "message": "Notification message (optional)"
}

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-bca-assay-calculator": {
      "name": "bca-assay-calculator",
      "description": "Least-squares A562 curve fit, back-calculated unknowns with dilution factor, extrapolation flags, and detergent/reducing-agent compatibility notes. Educational use only.",
      "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=bca-assay-calculator",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "bca-assay-calculator",
    "arguments": {
  "standards": "0, 0.000\n200, 0.200\n500, 0.500\n1000, 1.000\n2000, 2.000",
  "unknowns": "0.735\n1.640",
  "dilutionFactor": 1,
  "decimalPlaces": 2
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

Browse tools Open tool

curl -X POST 'https://api.elysiatools.com/en/api/tools/bca-assay-calculator' \ -H 'Content-Type: application/json' \ -d '{"standards":"0, 0.000\n200, 0.200\n500, 0.500\n1000, 1.000\n2000, 2.000","unknowns":"0.735\n1.640","dilutionFactor":1,"decimalPlaces":2}'

Name

Type

Required

Description

standards

textarea

Yes

One standard per line: concentration in µg/mL, then A562 (comma, semicolon, tab, or space separated).

unknowns

textarea

Yes

One sample A562 reading per line.

dilutionFactor

number

Yes

Dilution applied to the sample before the assay (1 = undiluted).

decimalPlaces

number

—

{ "mcpServers": { "elysiatools-bca-assay-calculator": { "name": "bca-assay-calculator", "description": "Least-squares A562 curve fit, back-calculated unknowns with dilution factor, extrapolation flags, and detergent/reducing-agent compatibility notes. Educational use only.", "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=bca-assay-calculator", "command": "", "args": [], "env": {}, "isActive": true, "type": "sse" } } }

{ "jsonrpc": "2.0", "id": 2, "method": "tools/call", "params": { "name": "bca-assay-calculator", "arguments": { "standards": "0, 0.000\n200, 0.200\n500, 0.500\n1000, 1.000\n2000, 2.000", "unknowns": "0.735\n1.640", "dilutionFactor": 1, "decimalPlaces": 2 } } }