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Health

OD600 to CFU/mL Converter (Calibration Curve Slope)

Convert OD600 to CFU/mL via a calibration slope with organism presets, blank correction, dilution handling, and linearity warnings.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/bacterial-growth-od' \
  -H 'Content-Type: application/json' \
  -d '{"od600":0.45,"blankOd":0.02,"organism":"ecoli","customFactor":0,"sampleDilution":1,"cultureVolumeMl":0}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP
POST https://api.elysiatools.com/en/api/tools/bacterial-growth-od

Parameters

NameTypeRequiredDescription
od600numberYesRaw absorbance reading of the (possibly diluted) sample at 600 nm.
blankOdnumberNoAbsorbance of sterile medium; subtracted from the reading.
organismselectYes—
customFactornumberNoYour own calibration slope (CFU/mL at OD600 = 1); used when the preset is Custom.
sampleDilutionnumberYes

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json
{
  "mcpServers": {
    "elysiatools-bacterial-growth-od": {
      "name": "bacterial-growth-od",
      "description": "Convert OD600 to CFU/mL via a calibration slope with organism presets, blank correction, dilution handling, and linearity warnings.",
      "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=bacterial-growth-od",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list
{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call
{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "bacterial-growth-od",
    "arguments": {
  "od600": 0.45,
  "blankOd": 0.02,
  "organism": "ecoli",
  "customFactor": 0,
  "sampleDilution": 1,
  "cultureVolumeMl": 0
}
  }
}
Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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Fold-dilution of the sample before reading (1 = undiluted).
cultureVolumeMlnumberNoLeave empty to skip the total-CFU calculation.

Response format

JSON result

JSON
{
  "key": {...},
  "metadata": {
    "key": "value"
  },
  "error": "Error message (optional)",
  "message": "Notification message (optional)"
}