Education
Transfection Efficiency Calculator (GFP Positive Rate)
GFP reporter transfection scoring: efficiency = GFP+ / total × 100 with a Wilson 95% CI in single mode and per-replicate mean ± SD in replicate mode. Interpretation bands from excellent (≥70%) to low (<10%) with optimization hints. Wilson 1927 / Newcombe 1998 / Kim & Eberwine 2010 derived. Educational use only.
json· JSON resultOpen tool
Quick start
Call this tool from your code in three languages.
cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/transfection-efficiency' \
-H 'Content-Type: application/json' \
-d '{"mode":"single","totalCells":500,"gfpPositiveCells":150,"replicateTotals":"480, 510, 505","replicateGfp":"120, 155, 130","decimalPlaces":2}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
HTTP
POST https://api.elysiatools.com/en/api/tools/transfection-efficiencyParameters
| Name | Type | Required | Description |
|---|---|---|---|
| mode | select | Yes | One pooled count, or several replicate wells/fields for mean ± SD. |
| totalCells | number | Yes | Total viable cells scored (single mode). |
| gfpPositiveCells | number | Yes | Cells scoring above the GFP threshold (single mode). |
| replicateTotals | text | Yes | Total cells per replicate well/field (replicate mode; same count as the GFP list). |
| replicateGfp | text |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
mcp.json
{
"mcpServers": {
"elysiatools-transfection-efficiency": {
"name": "transfection-efficiency",
"description": "GFP reporter transfection scoring: efficiency = GFP+ / total × 100 with a Wilson 95% CI in single mode and per-replicate mean ± SD in replicate mode. Interpretation bands from excellent (≥70%) to low (<10%) with optimization hints. Wilson 1927 / Newcombe 1998 / Kim & Eberwine 2010 derived. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=transfection-efficiency",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
tools/list
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
tools/call
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "transfection-efficiency",
"arguments": {
"mode": "single",
"totalCells": 500,
"gfpPositiveCells": 150,
"replicateTotals": "480, 510, 505",
"replicateGfp": "120, 155, 130",
"decimalPlaces": 2
}
}
}Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).