Education
RNA Concentration Calculator (A260, 1 OD = 40 µg/mL)
Beer–Lambert RNA quantitation: c = A260 × dilution factor × 40 / path length, plus ng/µL, total yield, a 0.1–1.5 linear-range check, and the RNA OD260/280 ≈ 2.0 purity reminder. Derived from Marmur & Doty, Sambrook & Russell, Manchester 1995, Thermo Fisher NanoDrop notes. Educational use only.
json· JSON resultOpen tool
Quick start
Call this tool from your code in three languages.
cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/rna-concentration-a260' \
-H 'Content-Type: application/json' \
-d '{"a260":0.4,"dilutionFactor":5,"pathLengthCm":1,"sampleVolumeUl":250,"decimalPlaces":2}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
HTTP
POST https://api.elysiatools.com/en/api/tools/rna-concentration-a260Parameters
| Name | Type | Required | Description |
|---|---|---|---|
| a260 | number | Yes | Measured absorbance of the (diluted) sample at 260 nm, blanked against the RNase-free elution buffer. |
| dilutionFactor | number | Yes | Sample-to-total dilution before measurement (1 = measured undiluted). |
| pathLengthCm | number | Yes | Cuvette path length (1 cm standard; microvolume readers may use 0.1–1 cm). |
| sampleVolumeUl | number | No | Total elution volume in µL; enter 0 to skip the total-yield calculation. |
| decimalPlaces |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
mcp.json
{
"mcpServers": {
"elysiatools-rna-concentration-a260": {
"name": "rna-concentration-a260",
"description": "Beer–Lambert RNA quantitation: c = A260 × dilution factor × 40 / path length, plus ng/µL, total yield, a 0.1–1.5 linear-range check, and the RNA OD260/280 ≈ 2.0 purity reminder. Derived from Marmur & Doty, Sambrook & Russell, Manchester 1995, Thermo Fisher NanoDrop notes. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=rna-concentration-a260",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
tools/list
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
tools/call
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "rna-concentration-a260",
"arguments": {
"a260": 0.4,
"dilutionFactor": 5,
"pathLengthCm": 1,
"sampleVolumeUl": 250,
"decimalPlaces": 2
}
}
}Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).