Education

RNA Concentration Calculator (A260, 1 OD = 40 µg/mL)

Beer–Lambert RNA quantitation: c = A260 × dilution factor × 40 / path length, plus ng/µL, total yield, a 0.1–1.5 linear-range check, and the RNA OD260/280 ≈ 2.0 purity reminder. Derived from Marmur & Doty, Sambrook & Russell, Manchester 1995, Thermo Fisher NanoDrop notes. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'http://127.0.0.1:3003/en/api/tools/rna-concentration-a260' \
  -H 'Content-Type: application/json' \
  -d '{"a260":0.4,"dilutionFactor":5,"pathLengthCm":1,"sampleVolumeUl":250,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST http://127.0.0.1:3003/en/api/tools/rna-concentration-a260

Parameters

Name	Type	Required	Description
a260	number	Yes	Measured absorbance of the (diluted) sample at 260 nm, blanked against the RNase-free elution buffer.
dilutionFactor	number	Yes	Sample-to-total dilution before measurement (1 = measured undiluted).
pathLengthCm	number	Yes	Cuvette path length (1 cm standard; microvolume readers may use 0.1–1 cm).
sampleVolumeUl	number	No	Total elution volume in µL; enter 0 to skip the total-yield calculation.
decimalPlaces	number

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-rna-concentration-a260": {
      "name": "rna-concentration-a260",
      "description": "Beer–Lambert RNA quantitation: c = A260 × dilution factor × 40 / path length, plus ng/µL, total yield, a 0.1–1.5 linear-range check, and the RNA OD260/280 ≈ 2.0 purity reminder. Derived from Marmur & Doty, Sambrook & Russell, Manchester 1995, Thermo Fisher NanoDrop notes. Educational use only.",
      "baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=rna-concentration-a260",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "rna-concentration-a260",
    "arguments": {
  "a260": 0.4,
  "dilutionFactor": 5,
  "pathLengthCm": 1,
  "sampleVolumeUl": 250,
  "decimalPlaces": 2
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

Browse tools Open tool