Education
Theoretical pI via the simplified ExPASy/Bjellqvist pKa set with bisection to zero net charge, plus net charge at pH 7.4, ProtParam average molecular weight, and charged composition. Bjellqvist 1993 / Gasteiger 2003 derived. Educational use only.
Call this tool from your code in three languages.
curl -X POST 'http://127.0.0.1:3003/en/api/tools/protein-isoelectric-point' \
-H 'Content-Type: application/json' \
-d '{"sequence":"GIVEQCCTSICSLYQLENYCN","decimalPlaces":2}'Send a POST request with your inputs as JSON. File parameters require a separate upload first.
POST http://127.0.0.1:3003/en/api/tools/protein-isoelectric-point| Name | Type | Required | Description |
|---|---|---|---|
| sequence | textarea | Yes | One-letter amino-acid codes, 20 standard residues only (FASTA headers, whitespace, and '*' are stripped). |
| decimalPlaces | number | No | — |
JSON result
{
"key": {...},
"metadata": {
"key": "value"
},
"error": "Error message (optional)",
"message": "Notification message (optional)"
}Add this tool to your Model Context Protocol server so AI agents can list and call it.
Add this block to your MCP client configuration:
{
"mcpServers": {
"elysiatools-protein-isoelectric-point": {
"name": "protein-isoelectric-point",
"description": "Theoretical pI via the simplified ExPASy/Bjellqvist pKa set with bisection to zero net charge, plus net charge at pH 7.4, ProtParam average molecular weight, and charged composition. Bjellqvist 1993 / Gasteiger 2003 derived. Educational use only.",
"baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=protein-isoelectric-point",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}After connecting to the SSE endpoint, list the exposed tools:
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Invoke the tool by its id, passing arguments built from its parameters:
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "protein-isoelectric-point",
"arguments": {
"sequence": "GIVEQCCTSICSLYQLENYCN",
"decimalPlaces": 2
}
}
}Questions or issues? Contact [email protected]