Education

OD260/230 Nucleic Acid Purity Ratio Calculator

Check salt/organic-solvent contamination with A260/A230 (pure 2.0–2.2; below 2.0 = guanidinium/phenol/EDTA/salt carryover from TRIzol or columns), with an optional A260/280 cross-check. Derived from Thermo Fisher T042, Sambrook & Russell, Manchester 1995. Educational use only.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'http://127.0.0.1:3003/en/api/tools/od260-230-ratio' \
  -H 'Content-Type: application/json' \
  -d '{"nucleicAcidType":"DNA","a260":0.5,"a230":0.25,"a280":0.278,"decimalPlaces":2}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST http://127.0.0.1:3003/en/api/tools/od260-230-ratio

Parameters

Name	Type	Required	Description
nucleicAcidType	select	Yes	260/230 expectations are identical for DNA and RNA; the type only tunes the optional A260/280 cross-check.
a260	number	Yes	Absorbance at 260 nm (nucleic acid peak), same diluted sample as the A230 reading.
a230	number	Yes	Absorbance at 230 nm (guanidine/phenol/salt peak) of the same diluted sample.
a280	number	No	Absorbance at 280 nm; enter 0 to skip the A260/280 cross-check.
decimalPlaces	number

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-od260-230-ratio": {
      "name": "od260-230-ratio",
      "description": "Check salt/organic-solvent contamination with A260/A230 (pure 2.0–2.2; below 2.0 = guanidinium/phenol/EDTA/salt carryover from TRIzol or columns), with an optional A260/280 cross-check. Derived from Thermo Fisher T042, Sambrook & Russell, Manchester 1995. Educational use only.",
      "baseUrl": "http://127.0.0.1:3003/mcp/sse?toolId=od260-230-ratio",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "od260-230-ratio",
    "arguments": {
  "nucleicAcidType": "DNA",
  "a260": 0.5,
  "a230": 0.25,
  "a280": 0.278,
  "decimalPlaces": 2
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

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