Education
Ligation Insert:Vector Molar Ratio Calculator
NEB-style ligation setup: solve mode computes insert_ng = ratio × vector_ng × (insert bp/vector bp); check mode reports the actual pmol ratio. Recommended bands by insert size (5–10:1 under 200 bp, 3–5:1 to 1 kb, 1–5:1 above), concatemer warning above 10:1, and nM concentrations from the reaction volume. NEB / Promega / Sambrook derived. Educational use only.
Quick start
Call this tool from your code in three languages.
curl -X POST 'https://api.elysiatools.com/en/api/tools/ligation-molar-ratio' \
-H 'Content-Type: application/json' \
-d '{"mode":"solveInsert","vectorSizeBp":3000,"vectorMassNg":100,"insertSizeBp":1000,"insertMassNg":50,"ratio":3,"reactionVolumeUl":20,"decimalPlaces":3}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
POST https://api.elysiatools.com/en/api/tools/ligation-molar-ratioParameters
| Name | Type | Required | Description |
|---|---|---|---|
| mode | select | Yes | Solve for the insert mass a desired ratio needs, or check the actual ratio of two masses. |
| vectorSizeBp | number | Yes | Double-digested vector backbone length in base pairs. |
| vectorMassNg | number | Yes | Mass of the linearized vector in the reaction. |
| insertSizeBp | number | Yes | Insert fragment length in base pairs. |
| insertMassNg | number | Yes |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
{
"mcpServers": {
"elysiatools-ligation-molar-ratio": {
"name": "ligation-molar-ratio",
"description": "NEB-style ligation setup: solve mode computes insert_ng = ratio × vector_ng × (insert bp/vector bp); check mode reports the actual pmol ratio. Recommended bands by insert size (5–10:1 under 200 bp, 3–5:1 to 1 kb, 1–5:1 above), concatemer warning above 10:1, and nM concentrations from the reaction volume. NEB / Promega / Sambrook derived. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=ligation-molar-ratio",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "ligation-molar-ratio",
"arguments": {
"mode": "solveInsert",
"vectorSizeBp": 3000,
"vectorMassNg": 100,
"insertSizeBp": 1000,
"insertMassNg": 50,
"ratio": 3,
"reactionVolumeUl": 20,
"decimalPlaces": 3
}
}
}