Education
Enzyme Turnover Number Calculator (kcat)
kcat = Vmax ÷ [E]total in min⁻¹ and s⁻¹, entered as enzyme amount, enzyme concentration, or protein concentration + molecular weight; bands results from very slow to catalase-class. Educational use only.
json· JSON resultOpen tool
Quick start
Call this tool from your code in three languages.
cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/enzyme-turnover-number' \
-H 'Content-Type: application/json' \
-d '{"mode":"amount","vmaxUmolMin":12,"enzymeUmol":0.02,"vmaxUMLMin":40,"enzymeUM":2,"proteinMgPerMl":1,"molecularWeightKda":50,"decimalPlaces":4}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
HTTP
POST https://api.elysiatools.com/en/api/tools/enzyme-turnover-numberParameters
| Name | Type | Required | Description |
|---|---|---|---|
| mode | select | Yes | How you know the enzyme: as an amount, a concentration, or protein concentration plus molecular weight. |
| vmaxUmolMin | number | Yes | Maximal velocity from a saturated assay (amount mode). |
| enzymeUmol | number | Yes | Total enzyme (better: active-site) amount in the assay (amount mode). |
| vmaxUMLMin | number | Yes | Maximal velocity as a concentration rate (concentration and protein modes). |
| enzymeUM | number |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
mcp.json
{
"mcpServers": {
"elysiatools-enzyme-turnover-number": {
"name": "enzyme-turnover-number",
"description": "kcat = Vmax ÷ [E]total in min⁻¹ and s⁻¹, entered as enzyme amount, enzyme concentration, or protein concentration + molecular weight; bands results from very slow to catalase-class. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=enzyme-turnover-number",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
tools/list
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
tools/call
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "enzyme-turnover-number",
"arguments": {
"mode": "amount",
"vmaxUmolMin": 12,
"enzymeUmol": 0.02,
"vmaxUMLMin": 40,
"enzymeUM": 2,
"proteinMgPerMl": 1,
"molecularWeightKda": 50,
"decimalPlaces": 4
}
}
}Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).
Questions or issues? Contact [email protected]