Education
Enzyme Inhibition Ki Calculator (Cheng–Prusoff IC50 Conversion)
IC50 ↔ Ki conversion for competitive, uncompetitive, and pure noncompetitive inhibition with mechanism notes, potency bands, and tight-binding caveats. Educational use only.
json· JSON resultOpen tool
Quick start
Call this tool from your code in three languages.
cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/enzyme-inhibition-ki' \
-H 'Content-Type: application/json' \
-d '{"mode":"ic50-to-ki","inhibitionType":"competitive","ic50Value":100,"kiValue":20,"valueUnit":"nano","substrateConc":50,"kmValue":25,"substrateUnit":"micro","decimalPlaces":2}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
HTTP
POST https://api.elysiatools.com/en/api/tools/enzyme-inhibition-kiParameters
| Name | Type | Required | Description |
|---|---|---|---|
| mode | select | Yes | Derive Ki from a measured IC50, or predict the IC50 a known Ki will give at your substrate concentration. |
| inhibitionType | select | Yes | Mechanism of inhibition — it decides which Cheng–Prusoff variant applies. |
| ic50Value | number | Yes | Measured IC50 in the unit selected below (IC50 → Ki mode). |
| kiValue | number | Yes | Known inhibition constant in the unit selected below (Ki → IC50 mode). |
| valueUnit | select |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
mcp.json
{
"mcpServers": {
"elysiatools-enzyme-inhibition-ki": {
"name": "enzyme-inhibition-ki",
"description": "IC50 ↔ Ki conversion for competitive, uncompetitive, and pure noncompetitive inhibition with mechanism notes, potency bands, and tight-binding caveats. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=enzyme-inhibition-ki",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
tools/list
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
tools/call
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "enzyme-inhibition-ki",
"arguments": {
"mode": "ic50-to-ki",
"inhibitionType": "competitive",
"ic50Value": 100,
"kiValue": 20,
"valueUnit": "nano",
"substrateConc": 50,
"kmValue": 25,
"substrateUnit": "micro",
"decimalPlaces": 2
}
}
}Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).
Questions or issues? Contact [email protected]