Health

Cell Confluency Estimator (Microscopy Grid Method)

Estimate adherent-cell confluency with the grid-count method: confluency% = occupied/total squares × 100 per field, with multi-field mean ± SD and optional time-to-target from the doubling time.

json· JSON resultOpen tool

Quick start

Call this tool from your code in three languages.

cURL

curl -X POST 'https://api.elysiatools.com/en/api/tools/confluency-estimator' \
  -H 'Content-Type: application/json' \
  -d '{"fieldCounts":"62, 58, 66","totalSquares":100,"doublingTimeHours":24,"targetConfluence":90}'

API reference

Send a POST request with your inputs as JSON. File parameters require a separate upload first.

Endpoint

HTTP

POST https://api.elysiatools.com/en/api/tools/confluency-estimator

Parameters

Name	Type	Required	Description
fieldCounts	textarea	Yes	One occupied-square count per field, separated by commas, spaces, or new lines.
totalSquares	number	Yes	Total squares in the counting grid overlaid on each field (10×10 = 100).
doublingTimeHours	number	No	Leave empty to skip the time-to-target estimate.
targetConfluence	number	Yes	Desired confluency for the time-to-target estimate.

Response format

JSON result

MCP integration

Add this tool to your Model Context Protocol server so AI agents can list and call it.

Server configuration

Add this block to your MCP client configuration:

mcp.json

{
  "mcpServers": {
    "elysiatools-confluency-estimator": {
      "name": "confluency-estimator",
      "description": "Estimate adherent-cell confluency with the grid-count method: confluency% = occupied/total squares × 100 per field, with multi-field mean ± SD and optional time-to-target from the doubling time.",
      "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=confluency-estimator",
      "command": "",
      "args": [],
      "env": {},
      "isActive": true,
      "type": "sse"
    }
  }
}

List available tools

After connecting to the SSE endpoint, list the exposed tools:

tools/list

{
  "jsonrpc": "2.0",
  "id": 1,
  "method": "tools/list"
}

Call this tool

Invoke the tool by its id, passing arguments built from its parameters:

tools/call

{
  "jsonrpc": "2.0",
  "id": 2,
  "method": "tools/call",
  "params": {
    "name": "confluency-estimator",
    "arguments": {
  "fieldCounts": "62, 58, 66",
  "totalSquares": 100,
  "doublingTimeHours": 24,
  "targetConfluence": 90
}
  }
}

Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).

Questions or issues? Contact [email protected]

Browse tools Open tool

curl -X POST 'https://api.elysiatools.com/en/api/tools/confluency-estimator' \ -H 'Content-Type: application/json' \ -d '{"fieldCounts":"62, 58, 66","totalSquares":100,"doublingTimeHours":24,"targetConfluence":90}'

Name

Type

Required

Description

fieldCounts

textarea

Yes

One occupied-square count per field, separated by commas, spaces, or new lines.

totalSquares

number

Yes

Total squares in the counting grid overlaid on each field (10×10 = 100).

doublingTimeHours

number

Leave empty to skip the time-to-target estimate.

targetConfluence

number

Yes

Desired confluency for the time-to-target estimate.

{ "mcpServers": { "elysiatools-confluency-estimator": { "name": "confluency-estimator", "description": "Estimate adherent-cell confluency with the grid-count method: confluency% = occupied/total squares × 100 per field, with multi-field mean ± SD and optional time-to-target from the doubling time.", "baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=confluency-estimator", "command": "", "args": [], "env": {}, "isActive": true, "type": "sse" } } }

{ "jsonrpc": "2.0", "id": 2, "method": "tools/call", "params": { "name": "confluency-estimator", "arguments": { "fieldCounts": "62, 58, 66", "totalSquares": 100, "doublingTimeHours": 24, "targetConfluence": 90 } } }