Education
Trypan Blue Cell Viability Calculator
Viability % and live/dead/total cells per mL from trypan blue exclusion counts, with culture-health interpretation. Educational use only.
json· JSON resultOpen tool
Quick start
Call this tool from your code in three languages.
cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/cell-viability-trypan-blue' \
-H 'Content-Type: application/json' \
-d '{"liveCells":180,"deadCells":20,"dilutionFactor":2,"squaresCount":4,"decimalPlaces":2}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
HTTP
POST https://api.elysiatools.com/en/api/tools/cell-viability-trypan-blueParameters
| Name | Type | Required | Description |
|---|---|---|---|
| liveCells | number | Yes | Bright, dye-excluding cells counted. |
| deadCells | number | Yes | Deep-blue, dye-permeable cells counted. |
| dilutionFactor | number | Yes | Total-to-sample volume ratio of the dye mix (1:1 with trypan blue ⇒ 2). |
| squaresCount | number | Yes | Number of 1 mm² large squares the counts cover. |
| decimalPlaces | number | No |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
mcp.json
{
"mcpServers": {
"elysiatools-cell-viability-trypan-blue": {
"name": "cell-viability-trypan-blue",
"description": "Viability % and live/dead/total cells per mL from trypan blue exclusion counts, with culture-health interpretation. Educational use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=cell-viability-trypan-blue",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
tools/list
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
tools/call
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "cell-viability-trypan-blue",
"arguments": {
"liveCells": 180,
"deadCells": 20,
"dilutionFactor": 2,
"squaresCount": 4,
"decimalPlaces": 2
}
}
}Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).
Questions or issues? Contact [email protected]