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PCR Annealing Temperature Calculator (Ta = Tm − 5°C)
PCR Ta by the simple rule (lower primer Tm − 5°C) or the Rychlik 1990 formula (0.3·Tm_primer + 0.7·Tm_product − 14.9), with a ±3°C gradient window and primer-Tm mismatch warning. Derived from Rychlik 1990, Innis & Gelfand 1990, Lorenz 2012. Lab-planning use only.
json· JSON resultOpen tool
Quick start
Call this tool from your code in three languages.
cURL
curl -X POST 'https://api.elysiatools.com/en/api/tools/annealing-temperature-calculator' \
-H 'Content-Type: application/json' \
-d '{"rule":"simple","forwardTm":62,"reverseTm":60,"productTm":85,"decimalPlaces":1}'API reference
Send a POST request with your inputs as JSON. File parameters require a separate upload first.
Endpoint
HTTP
POST https://api.elysiatools.com/en/api/tools/annealing-temperature-calculatorParameters
| Name | Type | Required | Description |
|---|---|---|---|
| rule | select | Yes | Simple rule uses the lower primer Tm; Rychlik also weighs the product Tm. |
| forwardTm | number | Yes | Melting temperature of the forward primer. |
| reverseTm | number | Yes | Melting temperature of the reverse primer. |
| productTm | number | Yes | Amplicon melting temperature (typically 75–90°C for a 100–500 bp product); used by the Rychlik formula. |
| decimalPlaces | number |
MCP integration
Add this tool to your Model Context Protocol server so AI agents can list and call it.
Server configuration
Add this block to your MCP client configuration:
mcp.json
{
"mcpServers": {
"elysiatools-annealing-temperature-calculator": {
"name": "annealing-temperature-calculator",
"description": "PCR Ta by the simple rule (lower primer Tm − 5°C) or the Rychlik 1990 formula (0.3·Tm_primer + 0.7·Tm_product − 14.9), with a ±3°C gradient window and primer-Tm mismatch warning. Derived from Rychlik 1990, Innis & Gelfand 1990, Lorenz 2012. Lab-planning use only.",
"baseUrl": "https://api.elysiatools.com/mcp/sse?toolId=annealing-temperature-calculator",
"command": "",
"args": [],
"env": {},
"isActive": true,
"type": "sse"
}
}
}List available tools
After connecting to the SSE endpoint, list the exposed tools:
tools/list
{
"jsonrpc": "2.0",
"id": 1,
"method": "tools/list"
}Call this tool
Invoke the tool by its id, passing arguments built from its parameters:
tools/call
{
"jsonrpc": "2.0",
"id": 2,
"method": "tools/call",
"params": {
"name": "annealing-temperature-calculator",
"arguments": {
"rule": "simple",
"forwardTm": 62,
"reverseTm": 60,
"productTm": 85,
"decimalPlaces": 1
}
}
}Chain multiple tools in one session with a comma-separated toolId list, e.g. /mcp/sse?toolId=png-to-webp,jpg-to-webp,gif-to-webp (max 20).