Absorbance Quantification and Acid-Base Calculations
Turn instrument readings into concentrations you can defend — convert between absorbance and transmittance, solve the Beer-Lambert law for whichever variable is missing, walk the nucleic acid chain from A260 to micrograms to nanomolar to copies per microliter, predict pH from strong acid and base concentrations, design buffers with Henderson-Hasselbalch, and check dissociation through molar conductivity.