# Henderson-Hasselbalch Buffer Recipe & Concentration Table Pick one of eight bench buffer systems, enter target pH, concentration and volume, and get the Henderson-Hasselbalch acid/salt split with weighable masses, temperature-corrected pKa, buffer capacity and ionic-strength estimate. > Canonical page: https://elysiatools.com/en/tools/henderson-hasselbalch-buffer-recipe-concentration-table - **Category:** Health - **Keywords:** henderson hasselbalch, buffer recipe, pka, phosphate buffer, tris buffer, ionic strength ## Overview The Henderson-Hasselbalch Buffer Recipe & Concentration Table calculator determines precise acid and base component ratios, weighable reagent masses, temperature-adjusted pKa values, buffer capacity, and ionic strength across eight standard bench buffer systems. ## Inputs - **Buffer system** (select) - **Target pH** (number) - **Total buffer concentration (mM)** (number) - **Final volume (mL)** (number) - **Working temperature (°C)** (number) ## When to use - Preparing biological or biochemical buffers with exact target pH, concentration, and volume requirements. - Formulating cold-room assays or high-temperature reactions where temperature-dependent pKa shifts alter buffer ratios. - Estimating solution ionic strength and buffer capacity to avoid salt interference in downstream enzymatic assays. ## How it works - Select your target buffer system from eight bench options, including Phosphate, Tris, HEPES, MES, MOPS, Acetate, Citrate, or Carbonate. - Enter the target pH, desired molar concentration in mM, total final preparation volume in mL, and planned working temperature in °C. - The tool applies temperature coefficients to compute the effective pKa and calculates the equilibrium acid-to-base ratio via the Henderson-Hasselbalch equation. - Review the resulting concentration table displaying individual component molarities, required dry masses to weigh out, buffer capacity (β), and estimated ionic strength. ## Use cases - Formulating Sørensen phosphate buffer stock solutions for physiological cell assays and histology washes. - Calculating Tris-HCl base and acid salt weights adjusted for 4 °C protein purification chromatography protocols. - Mixing HEPES or MOPS biological buffers at specified ionic strengths for cell culture and enzyme kinetics. ## Frequently asked questions ### Which buffer systems are supported by this calculator? The calculator supports eight systems: Acetate, Citrate, MES, Phosphate (Sørensen pair), MOPS, HEPES, Tris, and Bicarbonate/Carbonate. ### How does working temperature affect the buffer recipe? Buffer pKa values shift with temperature according to system-specific temperature coefficients, altering the required base-to-acid ratio. ### What is buffer capacity (β)? Buffer capacity measures resistance to pH change per mole of added acid or base, reaching its maximum when target pH equals the system pKa. ### Does the calculator account for ionic strength? Yes, it estimates total ionic strength based on the dissociation charges and equilibrium concentrations of the conjugate acid-base species. ### What is the recommended buffering range for these systems? 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