# DNA Copy Number Calculator (Concentration → Copies) Convert DNA mass concentration to copies/µL via copies = c(g/µL) × Avogadro ÷ (bp × 660), with ng/µL–pg/µL–µg/µL units, pmol/µL output, total copies per volume, and A260-overestimation caveats. Lab-planning use only. > Canonical page: https://elysiatools.com/en/tools/dna-copy-number-calculator - **Category:** Education - **Keywords:** dna copy number calculator, copies per microliter, dna concentration to copies, avogadro dna, plasmid copy number, pcr template copies, qpcr standard curve, molar concentration dna, pmol/µl dna, molecular weight dna, molecular biology, lab calculator ## Overview The DNA Copy Number Calculator converts DNA concentration and template length into copies per µL using the average molecular weight of 660 g/mol per base pair and Avogadro’s constant. It also reports pmol/µL and, when a sample volume is entered, the total number of DNA copies. ## Inputs - **DNA Concentration** (number): Measured DNA concentration in the selected unit. - **Concentration Unit** (select): ng/µL and µg/mL are numerically identical; pg/µL is the Qubit range. - **Template Length (bp)** (number): Total double-stranded length in bp: plasmid size, genome size, or amplicon length. - **Sample Volume (µL, optional)** (number): Volume of template added (e.g. per PCR reaction); enter 0 to skip the total-copies calculation. - **Decimal Places** (number) ## When to use - Estimate plasmid, genome, or amplicon copies per µL from a measured DNA concentration. - Calculate how many template molecules are present in a selected sample volume. - Prepare DNA standards or plan PCR and qPCR template amounts. ## How it works - Enter the DNA concentration and select ng/µL, pg/µL, µg/mL, or µg/µL. - Enter the total double-stranded template length in base pairs. - Optionally enter the template volume in µL to calculate total copies. - The calculator applies copies = concentration in g/µL × Avogadro’s constant ÷ (base pairs × 660) and returns copies/µL and pmol/µL. ## Use cases - Convert plasmid miniprep measurements into template copies for PCR or qPCR planning. - Estimate molecular copies in lambda DNA or other long-template standards. - Compare copy concentrations across DNA samples reported in different mass units. ## Frequently asked questions ### What does this DNA calculator calculate? It converts DNA mass concentration into molecular copies per µL, pmol/µL, and optionally total copies in a specified volume. ### Which concentration units are supported? You can enter ng/µL, pg/µL, µg/mL, or µg/µL. ### What template length should I enter? Enter the total double-stranded length in base pairs, such as a plasmid size, genome size, or amplicon length. ### How is total copy number calculated? The calculator multiplies the copies per µL result by the optional sample volume in µL. Enter 0 to skip this calculation. ### Is the result suitable for exact oligonucleotide quantification? 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