# Trypan Blue Cell Viability Calculator Viability % and live/dead/total cells per mL from trypan blue exclusion counts, with culture-health interpretation. Educational use only. > Canonical page: https://elysiatools.com/en/tools/cell-viability-trypan-blue - **Category:** Education - **Keywords:** trypan blue viability calculator, cell viability calculation, live dead cell count, trypan blue exclusion assay, viability percentage formula, live cells per ml, cell culture health check, hemocytometer viability, cell counting viability, lab calculator ## Overview The Trypan Blue Cell Viability Calculator estimates cell viability, live and dead cell concentrations, and total cells per mL from live-cell and dead-cell counts, dilution factor, and hemocytometer square count. It also provides an educational interpretation of culture health. ## Inputs - **Live (Unstained) Cells** (number): Bright, dye-excluding cells counted. - **Dead (Blue) Cells** (number): Deep-blue, dye-permeable cells counted. - **Dilution Factor** (number): Total-to-sample volume ratio of the dye mix (1:1 with trypan blue ⇒ 2). - **Squares Counted** (number): Number of 1 mm² large squares the counts cover. - **Decimal Places** (number) ## When to use - Check cell culture viability before passaging or starting an experiment. - Convert hemocytometer trypan blue counts into live and total cells per mL. - Quickly assess whether a culture appears healthy, acceptable, or stressed based on viability. ## How it works - Enter the number of bright, unstained live cells and blue, stained dead cells. - Enter the dilution factor and the number of 1 mm² large squares counted. - The calculator computes viability as live cells divided by total counted cells, multiplied by 100. - It reports cell concentrations per mL and an educational culture-health status. ## Use cases - Preparing a consistent cell suspension for passaging or seeding. - Comparing culture health after thawing, dissociation, or routine maintenance. - Recording live-cell concentration from manual trypan blue exclusion counts. ## Frequently asked questions ### What does the viability percentage measure? It is the percentage of counted cells that are live: live cells ÷ (live cells + dead cells) × 100. ### What dilution factor should I enter for a 1:1 trypan blue mix? Enter 2 because the final dye mixture contains one part sample and one part trypan blue. ### What does the squares count represent? It is the number of 1 mm² large hemocytometer squares included in the cell counts. ### How are live cells per mL calculated? The calculation uses the live-cell count, dilution factor, 10⁴, and the number of large squares counted. ### Is this calculator intended for clinical decisions? 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