# Amino Acid Composition Analyzer Per-residue counts and mol%, group tallies, Kyte–Doolittle GRAVY score, and ProtParam MW from a sequence. Educational use only. > Canonical page: https://elysiatools.com/en/tools/amino-acid-composition - **Category:** Education - **Keywords:** amino acid composition analyzer, protein sequence composition, amino acid percentage calculator, gravy score calculator, kyte doolittle hydropathy, protein hydropathy grand average, aromatic residue content, charged residue count, hydrophobicity protein sequence, biochemistry calculator, lab calculator ## Overview Amino Acid Composition Analyzer calculates residue counts and molar percentages from a protein sequence, along with group tallies, Kyte–Doolittle GRAVY hydropathy, and ProtParam molecular weight. Enter a one-letter amino-acid sequence and choose the number of decimal places for the JSON result. Educational use only. ## Inputs - **Amino Acid Sequence** (textarea): One-letter amino-acid sequence (case-insensitive; spaces and * - . separators are ignored). - **Decimal Places** (number) ## When to use - Check the amino-acid composition and percentage of residues in a protein sequence. - Estimate overall hydrophobicity with the Kyte–Doolittle GRAVY score. - Review residue groups and molecular weight during protein sequence analysis or experiment planning. ## How it works - Enter a one-letter amino-acid sequence; input is case-insensitive. - Spaces and separators such as *, - and . are ignored. - The analyzer counts residues, calculates molar percentages and group totals, and computes GRAVY and molecular weight. - Set decimal places from 0 to 8, then review the structured JSON output. ## Use cases - Compare composition and hydropathy across protein constructs or sequence regions. - Check affinity-tag sequences for residue enrichment and overall hydrophilicity. - Perform quick educational calculations for protein biochemistry and laboratory planning. ## Frequently asked questions ### What sequence format does the analyzer accept? Enter a one-letter amino-acid sequence. Uppercase and lowercase letters are accepted. ### Are spaces and separators allowed? Yes. Spaces and the separators *, - and . are ignored. ### What does the GRAVY score show? GRAVY is the average Kyte–Doolittle hydropathy score. Positive values indicate a hydrophobic tendency, while negative values indicate a hydrophilic tendency. ### Can I control the number of decimal places? Yes. Choose from 0 to 8 decimal places; the default is 2. ### How are ambiguous amino-acid codes treated? B, J, O, U, X and Z are counted separately and excluded from molar percentage calculations. ## Related tools - [Mean Platelet Volume (MPV) Analyzer](https://elysiatools.com/en/tools/mean-platelet-volume-analyzer): Analyze Mean Platelet Volume (MPV) in fL, an index of platelet production kinetics. Reference range ≈ 7.5–11.5 fL (lab- and method-dependent). Low MPV (< 7.5 fL, small old platelets) indicates suppressed marrow production (aplastic anemia, chemotherapy/radiation, recent platelet transfusion, iron-deficiency regeneration, Wiskott-Aldrich). High MPV (> 11.5 fL, large young platelets) indicates peripheral destruction with compensatory turnover or macrothrombocytopenia (immune thrombocytopenia ITP, post-splenectomy, myeloproliferative neoplasms, severe sepsis in recovery, Bernard-Soulier / MYH9, megaloblastic anemia; elevated MPV after acute MI marks platelet activation and adverse prognosis). Optionally enter the platelet count to refine the differential: low platelets + high MPV → peripheral destruction; low + low → underproduction; high + low → reactive thrombocytosis; high + high → myeloproliferative neoplasm. Pre-analytical caveat: platelets swell in EDTA over ~2 h raising MPV, and values are method-dependent (impedance > optical > immuno) — compare within the same lab and analyzer. 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